MERCK 111972.0500 Tsc Agar Tryptose Sulfite Cycloserine Agar (Base) For Microbiology

Key Spec Table

ph Value	Solubility
7.4 - 7.8 (H₂O, 25 °C)	42 g/l

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Description
Catalogue Number	111972
Synonyms	TSC-agar, Tryptose sulfite cycloserine agar
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ200

Physicochemical Information
pH value	7.4 - 7.8 (H₂O, 25 °C)
Bulk density	660 kg/m3
Solubility	42 g/l

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H319: Causes serious eye irritation.
Precautionary Statement(s)	P264: Wash skin thoroughly after handling.P280: Wear eye protection/ face protection.P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.P337 + P313: If eye irritation persists: Get medical advice/ attention.
Signal Word	Warning
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Specifications
Appearance (clearness)	clear
Appearance (color)	brown
Solidification behaviour (2 hrs., 45 °C)	liquid
pH-value (25 °C)	7.4 - 7.8
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Poured plate technique
Inoculum on reference medium (Clostridium perfringens ATCC 13124 (WDCM 00007))
Inoculum on reference medium (Clostridium perfringens ATCC 12916 (WDCM 00080))
Inoculum on reference medium (Clostridium perfringens ATCC 10543)
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003))	≥ 10000
Colony count (Clostridium perfringens ATCC 13124 (WDCM 00007))
Colony count (Clostridium perfringens ATCC 12916 (WDCM 00080))
Colony count (Clostridium perfringens ATCC 10543)
Recovery on test medium (Clostridium perfringens ATCC 13124 (WDCM 00007))	≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 12916 (WDCM 00080))	≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 10543)	≥ 70 %
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003))	total inhibition
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007))	+
Blacking (Clostridium perfringens ATCC 12916 (WDCM 00080))	+
Blacking (Clostridium perfringens ATCC 10543)	+
Incubation: 20 ± 2 hours at (37 ± 1) °C anaerobic
Membrane filtration
Inoculum on reference medium (Clostridium perfringens ATCC 13124 (WDCM 00007))
Inoculum on reference medium (Clostridium perfringens ATCC 12916 (WDCM 00080))
Inoculum on reference medium (Clostridium perfringens ATCC 10543)
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003))	≥ 10000
Colony count (Clostridium perfringens ATCC 13124 (WDCM 00007))
Colony count (Clostridium perfringens ATCC 12916 (WDCM 00080))
Colony count (Clostridium perfringens ATCC 10543)
Recovery on test medium (Clostridium perfringens ATCC 13124 (WDCM 00007))	≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 12916 (WDCM 00080))	≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 10543)	≥ 70 %
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003))	total inhibition
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007))	+
Blacking (Clostridium perfringens ATCC 12916 (WDCM 00080))	+
Blacking (Clostridium perfringens ATCC 10543)	+
Incubation: 21 ± 3 hours at (44 ± 1) °C anaerobicA recovery rate of 70 % is equivalent to a productivity value of 0.7.The indicated colony counts result from the sum of a triple determination.Reference media: TSC-Agar
Fluorescence (Clostridium perfringens ATCC 13124 (WDCM 00007))	+
Fluorescence (Clostridium perfringens ATCC 12916 (WDCM 00080))	+
Incubation: 20 hrs. 37 °C.Reference: TSC-agar for Cl.perfingens; Blood-agar for all other strains.