MERCK 110275.0500 Vrbd Agar Crystal-Violet Neutral-Red Bile Glucose Agar Acc. To Mossel For Microbiology

Key Spec Table

ph Value	Solubility
7.3 (40 g/l, H₂O, 37 °C) (after autoclaving)	40 g/l

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Description
Catalogue Number	110275
Synonyms	Crystal-violet neutral-red bile dextrose agar, VRBD-agar (Crystal-violet neutral-red bile dextrose agar) acc. to MOSSEL
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ200

Physicochemical Information
pH value	7.3 (40 g/l, H₂O, 37 °C) (after autoclaving)
Bulk density	720 kg/m3
Solubility	40 g/l

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Specifications
Appearance (clearness)	clear
Appearance (color)	red
Solidification behaviour (4 hrs., 45 °C)	liquid
pH-value (25 °C)	7.2 - 7.6
Typical composition (g/litre): Peptone from gelatine 7.0; Yeast extract 3.0; Sodium chloride 5.0; D(+)Glucose 10.0; Bile salt mixture 1.5; Neutralred 0.03; Crystal violet 0.002; Agar-agar 13.0.
Growth promotion test in accordance with the current version of harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	10 - 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	10 - 100
Inoculum (Staphylococcus aureus ATCC 6538 (WDCM 00032))	≥ 10000
Inoculum (Bacillus cereus ATCC 11778 (WDCM 00001))	≥ 10000
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))	50 - 150 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	50 - 150 %
Colony color (Escherichia coli ATCC 8739 (WDCM 00012))	red colonies
Colony color (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	nearly colourless to slightly red colonies
Precipitate (Escherichia coli ATCC 8739 (WDCM 00012))	+
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032))	no growth
Growth (Bacillus cereus ATCC 11778 (WDCM 00001))	no growth
Incubation:up to 18 hours at 30-35°C aerobicStaphylococcus aureus and Bacillus cereus at least 24 hours.Reference medium: Tryptic Soy Agar
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030))
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Escherichia coli ATCC 25922 (WDCM 00013))
Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031))
Colony count (Salmonella enteritidis ATCC 13076 (WDCM 00030))
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 50 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))	≥ 50 %
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))	≥ 50 %
Recovery on test medium (Salmonella enteritidis ATCC 13076 (WDCM 00030))	≥ 50 %
Colonies (Escherichia coli ATCC 8739 (WDCM 00012))	Pink to red colonies with or without precipitation halo
Colonies (Escherichia coli ATCC 25922 (WDCM 00013))	Pink to red colonies with or without precipitation halo
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031))	Pink to red colonies with or without precipitation halo
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030))	Pink to red colonies with or without precipitation halo
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087))	total inhibition
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009))	total inhibition
Incubation: 24 ± 2 hours at 37 ± 1 °CA recovery rate of 50 % is equivalent to a productivity value of 0.5.The indicated colony counts result from the sum of a triple determination.Reference medium: Tryptic Soy Agar
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.