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SD.M.109844.1000
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          Description
          Catalogue Number109844
          DescriptionEosin Y-solution 0.5% aqueous
          OverviewEosin Y-solution 0.5% aqueous - for microscopy, is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin. The hematoxylin and eosin (H&E) staining method is the method most frequently used for the staining of histology material. This Eosin Y-solution is a 0.5% aqueous staining solution, that can be used e. g. for the histological staining of instantaneous sections.The staining mechanism behind the H&E staining is a physico-chemical process. In the first step, the positively charged nuclear dye (hematoxylin) Mayer’s hemalum solution for microscopy(Cat.no. 109249) or Hematoxylin solution modified acc. to Gill III (Cat. no. 105174) binds to the negatively charged phosphate groups of the nucleic acid of the cell nucleus. The nuclei will be dyed dark blue to dark violet. The second step is the counterstaining with negatively charged anionic Eosin Y, a xanthene dye. Eosin binds to the positively charged plasma proteins. Cytoplasm and intercellular substances are stained pink to red, while erythrocytes will appear with yellow to orange color.The 500ml bottle provides 1500 stainings. It is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          Product Information
          HS Code3212 90 90
          Quality LevelMQ400
          Applications
          Applicationfor microscopy
          Physicochemical Information
          Density1.00 g/cm3
          Safety Information according to GHS
          Hazard Pictogram(s)
          Hazard Statement(s)H317: May cause an allergic skin reaction.
          Precautionary Statement(s)P261: Avoid breathing mist or vapours.P272: Contaminated work clothing should not be allowed out of the workplace.P280: Wear protective gloves.P302 + P352: IF ON SKIN: Wash with plenty of water.P333 + P313: If skin irritation or rash occurs: Get medical advice/ attention.P362 + P364: Take off contaminated clothing and wash it before reuse.
          Signal WordWarning
          Storage class10 - 13 Other liquids and solids
          WGKWGK 2 obviously hazardous to water
          Disposal28Aqueous solutions: Container D.
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Specifications
          Suitability for microscopy (Tissue section)passes test
          nucleidark blue to dark violet
          Cytoplasmpink to red
          intercellular substancespink to red
          Erythrocytesyellow to orange