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| Description |
|---|
| Catalogue Number | 109278 |
| Description | Wright's eosine methylene blue |
| Overview | Wright’s eosin methylene blue - for microscopy staining dye, is a defined mixture of the dyes C.I. 52015 and C.I. 45380 for hematological staining procedures and is used in conjunction with eosin Y. The Wright’s eosin methylene blue solution can be used for analysis and diagnostics in the whole blood differentiation. The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage. |
| Product Information |
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| HS Code | 3212 90 00 |
| Quality Level | MQ400 |
| Applications |
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| Application | for microscopy |
| Physicochemical Information |
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| Bulk density | 520 kg/m3 |
| Safety Information according to GHS |
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| Hazard Pictogram(s) | |
| Hazard Statement(s) | H317: May cause an allergic skin reaction.H319: Causes serious eye irritation. |
| Precautionary Statement(s) | P261: Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.P264: Wash skin thoroughly after handling.P272: Contaminated work clothing should not be allowed out of the workplace.P280: Wear protective gloves/ eye protection/ face protection.P302 + P352: IF ON SKIN: Wash with plenty of water.P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
| Signal Word | Warning |
| Storage class | 10 - 13 Other liquids and solids |
| WGK | WGK 3 highly hazardous to water |
| Disposal | 3Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C. |
| Safety Information |
|---|
| Categories of danger | harmful, irritant |
| Storage and Shipping Information |
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| Storage | Store at +5°C to +30°C. |
| Specifications |
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| Identity (UV/VIS-Spectrum) | passes test |
| pH-value (0,01 %; methanol 50 %) | 6.0 - 7.0 |
| Absorption maximum λ 1 (Methanol) | 648 - 651 nm |
| Absorption maximum λ 2 (Methanol) | 521 - 524 nm |
| Spec. Absorptivity A 1%/1cm- λ1max; 0.0005 %; methanol; calculated on dried substance | 1200 - 1400 |
| - λ2max; 0.0005 %; methanol; calculated on dried substance | 650 - 850 |
| TLC-Test | passes test |
| Loss on drying (105 °C) | ≤ 10 % |
| Suitability for microscopy | passes test |
