MERCK 109261.1000 Lugol´S Sol. Gr For Analysis
| Description |
|---|
| Catalogue Number | 109261 |
| Synonyms | Iodine-potassium iodide solution |
| Description | Lugol's solution (diluted iodine-potassium iodide solution) |
| Overview | Lugol’s solution (diluted iodine-potassium iodide solution - for the Gram staining method, is a ready-to-use solution used in Bacteriology, for example, for the staining of Gram bacteria. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet (Cat.no. 109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution), a dye-iodine complex will form. During the decolorizing step (Gram's decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Cat.no. 109217) and will then appear pink to red.Lugol's solution (diluted iodine-potassium iodide solution) is capable of staining up to 500 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage. |
| Product Information |
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| HS Code | 3212 90 90 |
| Quality Level | MQ400 |
| Applications |
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| Application | for the Gram staining method |
| Physicochemical Information |
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| Boiling point | 100 °C (1013 hPa) |
| Density | 1.01 g/cm3 (20 °C) |
| pH value | 3.5 (H₂O, 20 °C) |
| Safety Information according to GHS |
|---|
| Storage class | 10 - 13 Other liquids and solids |
| WGK | WGK 2 obviously hazardous to water |
| Storage and Shipping Information |
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| Storage | Store at +15°C to +25°C. |
| Specifications |
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| Suitability for microscopy | passes test |
| gram-positve microorganisms | blue-violet |
| gram-negative microorganisms | pink to red |
| Global Trade Item Number |
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| Catalogue Number | GTIN |
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| 1092611000 | 04022536112701 |
| 1092612500 | 04022536557410 |
