MERCK 109205.0500 Mtsb-Broth With Novobiocin For Microbiology

Key Spec Table

ph Value	Solubility
7.1 - 7.5 (33 g/l, H₂O, 25 °C) (after autoclaving)	33 g/l

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Description
Catalogue Number	109205

Product Information
HS Code	3821 00 00
Quality Level	MQ200

Physicochemical Information
pH value	7.1 - 7.5 (33 g/l, H₂O, 25 °C) (after autoclaving)
Solubility	33 g/l

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Specifications
Appearance (clearness)	clear
Appearance (color)	yellowish-brown
pH-value (25 °C)	7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.Mixed inoculum of E.coli and S.aureusAdditionaly monoculture of S.aureus
Inoculum on reference medium (Escherichia coli ATCC 35150 (O157))	≤ 100
Inoculum on reference medium (Escherichia coli ATCC 700728(WDCM 00014))	≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))	≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))	≥ 10000
Growth (Escherichia coli ATCC 35150 (O157))	> 10 yellowish-brown colonies on CT-SMAC agar
Growth (Escherichia coli ATCC 700728 (WDCM 00014))	> 10 yellowish-brown colonies on CT-SMAC agar
Growth Escherichia coli ATCC 25922 (WDCM 00013)	> 10 pink colonies on CT-SMAC agar
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034))	total inhibition on TSA
Incubation:mTSB broth: 18 - 24 hours at 41,5 °C, aerobicConfirmation on CT-SMAC Agar resp. TSA: 24 ± 3 hours at 37 ± 1 °C aerobic