MERCK 105458.0500 Tryptic Soy Agar Casein-Peptone Soymeal-Peptone Agar For Microbiology Usp

Key Spec Table

ph Value	Solubility
7.1 - 7.5 (40 g/l, H₂O, 25 °C) (after autoclaving)	40 g/l

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Description
Catalogue Number	105458
Replaces	1054585007
Synonyms	Casein-peptone soyameal-peptone agar, Cacein-peptone soyameal-peptone agar
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ200

Physicochemical Information
pH value	7.1 - 7.5 (40 g/l, H₂O, 25 °C) (after autoclaving)
Bulk density	620 kg/m3
Solubility	40 g/l

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H317: May cause an allergic skin reaction.
Precautionary Statement(s)	P261: Avoid breathing dust.P272: Contaminated work clothing should not be allowed out of the workplace.P280: Wear protective gloves.P302 + P352: IF ON SKIN: Wash with plenty of water.P333 + P313: If skin irritation or rash occurs: Get medical advice/ attention.P362 + P364: Take off contaminated clothing and wash it before reuse.
Signal Word	Warning
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Specifications
Appearance (clearness)	clear
Appearance (color)	yellowish brown
pH-value (25 °C)	7.1 - 7.5
Solidification behaviour (2 hrs., 45 °C)	liquid
Stability test (Color and hemolysis)	non-hemolytic
Typical composition (g/litre): Pancreatic digest of casein 15.0; Papaic digest of soya bean 5.0; Sodium chloride 5.0; Agar-Agar 15.0.
Groth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))	10 - 100
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003))	10 - 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	10 - 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	10 - 100
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054))	10 - 100
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053))	10 - 100
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032))
Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003))
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))
Colony count (Candida albicans ATCC 10231 (WDCM 00054))
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053))
Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))	≥ 70 %
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003))	≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 70 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	≥ 70 %
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054))	≥ 70 %
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053))	≥ 50 %
Incubation: 24 hrs.; 30-35°C; aerobic; C.albicans and Aasiliensis up to 5 daysReference media: blood agar and SABOURAUD-Agar for C.albicans and Aasiliensis
The information above is current at this time of publication and is subject to change without notice (exept for customers holding a change control agreement with our company). The information/format can be altered at any time and in any way if internal company matters or Standard Regulations do say so.
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001))
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021))
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Escherichia coli ATCC 25922 (WDCM 00013))
Colony count (Bacillus cereus ATCC 11778 (WDCM 00001))
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021))
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034))
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 70 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))	≥ 70 %
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001))	≥ 70 %
Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021))	≥ 70 %
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))	≥ 70 %
Incubation: 24 ± 3 hours at 37 ± 1 °CReference media: Tryptic Soy AgarA recovery rate of 70 % is equivalent to a productivity value of 0.7.The indicated colony counts result from the sum of a triple determination.