Key Spec Table
| ph Value | Solubility |
|---|
| 7.3 (45 g/l, H₂O, 37 °C) (after autoclaving) | 45 g/l |
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| Description |
|---|
| Catalogue Number | 105394 |
| Synonyms | MOSSEL Enterobacteriaceae enrichment broth, Enterobacteriaceae enrichment broth |
| Overview | GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly. |
| Product Information |
|---|
| HS Code | 3821 00 00 |
| Quality Level | MQ200 |
| Physicochemical Information |
|---|
| pH value | 7.3 (45 g/l, H₂O, 37 °C) (after autoclaving) |
| Bulk density | 630 kg/m3 |
| Solubility | 45 g/l |
| Safety Information according to GHS |
|---|
| Hazard Statement(s) | H412: Harmful to aquatic life with long lasting effects. |
| Precautionary Statement(s) | P273: Avoid release to the environment.P501: Dispose of contents/ container to an approved waste disposal plant. |
| Storage class | 10 - 13 Other liquids and solids |
| WGK | WGK 3 highly hazardous to water |
| Disposal | 3Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C. |
| Storage and Shipping Information |
|---|
| Storage | Store at +15°C to +25°C. |
| Specifications |
|---|
| Appearance (clearness) | clear |
| Appearance (color) | green |
| pH-value (25 °C) | 7.0 - 7.4 |
| Growth promotion test in accordance with the current version of DIN EN ISO 11133.Mixed inoculum E.coli resp. Salmonella and E.faecalis.Additionaly monoculture of E.faecalisConfirmation on VRBD agar resp. Tryptic soy agar |
| Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) | ≤ 100 |
| Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) | ≤ 100 |
| Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)) | ≤ 100 |
| Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)) | ≤ 100 |
| Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | ≥ 10000 |
| Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | ≥ 10000 |
| Growth on VRBD-Agar(Escherichia coli ATCC 8739 (WDCM 00012)) | > 10 colonies |
| Growth on VRBD-Agar(Escherichia coli ATCC 25922 (WDCM 00013)) | > 10 colonies |
| Growth on VRBD-Agar(Salmonella typhimurium ATCC 14028 (WDCM 00031)) | > 10 colonies |
| Growth on VRBD-Agar(Salmonella enteritidis ATCC 13076 (WDCM 00030)) | > 10 colonies |
| Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | total inhibition |
| Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | total inhibition |
| Colonies (Escherichia coli ATCC 8739 (WDCM 00012)) | pink to red colonies with or without precipitation halo |
| Colonies (Escherichia coli ATCC 25922 (WDCM 00013)) | pink to red colonies with or without precipitation halo |
| Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)) | pink to red colonies with or without precipitation halo |
| Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)) | pink to red colonies with or without precipitation halo |
| Incubation:EE broth: 24 ± 2 hours at 37 ± 1 °C aerobicConfirmation on VRBD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic |
