MERCK 100466.0500 Dichloran Rose-Bengal Chloramphenicol Agar (Drbc) acc. ISO 21527 and FDA-BAM GranuCult®

Key Spec Table

ph Value	Solubility
5.4 - 5.8 (32.6 g/l, H₂O, 25 °C)	32.6 g/l

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Description
Catalogue Number	100466
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ200

Physicochemical Information
pH value	5.4 - 5.8 (32.6 g/l, H₂O, 25 °C)
Solubility	32.6 g/l

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Specifications
Appearance (clearness)	clear to slightly opalescent
Appearance (color)	pink
pH-value (25 °C)	5.4 - 5.8
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058))
Colony count (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058))
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058))	≥ 50 %
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053))
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053))
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053))	≥ 50 %
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054))
Colony count (Candida albicans ATCC 10231 (WDCM 00054))
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054))	≥ 50 %
Inoculum on reference medium (Mucor racemosus ATCC 42647 (WDCM00181))
Colony count (Mucor racemosus ATCC 42647 (WDCM00181))
Recovery on test medium (Mucor racemosus ATCC 42647 (WDCM00181))	≥ 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012))	no growth
Growth Escherichia coli ATCC 25922 (WDCM 00013)	no growth
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003))	no growth
Incubation: 5 days at 25 ± 1 °CA recovery rate of 50 % is equivalent to a productivity value of 0.5.The indicated colony counts result from the sum of a triple determination.Reference media: SABOURAUD dextrose agar