MERCK 100092.0010 Fraser Listerya Ammonium Iron (III) Supplement

Key Spec Table

ph Value	Solubility
6 - 8 (100 g/l, H₂O, 20 °C)	1200 g/l

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Description
Catalogue Number	100092
Replaces	1000920017

Product Information
CAS number	1185-57-5
EC number	214-686-6
Hill Formula	C₆H₈O₇*xFe*xH₃N
HS Code	2918 15 00
Quality Level	MQ200

Applications
Application	FRASER Listeria Ammonium iron(III) Supplement. CAS 1185-57-5, pH 6 - 8 (100 g/l, H₂O, 20 °C).

Physicochemical Information
Density	1.064 g/cm3 (978 hPa, 26 °C)
pH value	6 - 8 (100 g/l, H₂O, 20 °C)
Solubility	1200 g/l

Toxicological Information
LD 50 oral	LD50 Rat > 2000 mg/kg

Safety Information according to GHS
RTECS	GE7540000
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Specifications
Growth promotion test according to ISO 11133Mixed inoculum of Listeria, E.coli and E.faecalisAdditionaly monoculture of E.coli and E.faecalisPre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser BrothConfirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium- Listeria monocytogenes ATCC 19111 (WDCM 00020)	10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013)	≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012)	≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	≥ 1000
Blacking- Listeria monocytogenes ATCC 19111 (WDCM 00020)	+
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	+
- Escherichia coli ATCC 25922 (WDCM 00013)	-
- Escherichia coli ATCC 8739 (WDCM 00012)	-
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	-
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	-
Growth on ALOA® medium- Listeria monocytogenes ATCC 19111 (WDCM 00020)	> 10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	> 10 CFU
Colonies- Listeria monocytogenes ATCC 19111 (WDCM 00020)	bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	bluegreen with non transparent zone
Growth on Tryptic soy agar- Escherichia coli ATCC 25922 (WDCM 00013)	no growth
- Escherichia coli ATCC 8739 (WDCM 00012)	no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	< 100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	< 100 CFU
Incubation:Half Fraser Broth: 24 hours at 30°C, aerobicFraser Broth: 48 hours at 37°C, aerobicConfirmation on ALOA®-Medium: 48 hours at 37°C, aerobicConfirmation on Tryptic soy agar: 24 hours at 35°C, aerobic