MERCK 100025.0500 Demi Fraser Broth With Antibiotics For Microbiology
| Description |
|---|
| Catalogue Number | 100025 |
| Overview | GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly. |
| Product Information |
|---|
| HS Code | 3821 00 00 |
| Quality Level | MQ200 |
| Physicochemical Information |
|---|
| pH value | 7.0 - 7.4 (55 g/l, H₂O, 25 °C) (after autoclaving) |
| Solubility | 55 g/l |
| Safety Information according to GHS |
|---|
| Storage class | 10 - 13 Other liquids and solids |
| WGK | WGK 3 highly hazardous to water |
| Disposal | 3Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C. |
| Storage and Shipping Information |
|---|
| Storage | Store at +15°C to +25°C. |
| Specifications |
|---|
| Appearance (clearness) | clear |
| Appearance (color) | yellowish-brown |
| pH-value (25 °C) | 7.0 - 7.4 |
| Growth promotion test in accordance with the current version of DIN EN ISO 11133.Mixed inoculum of Listeria, E.coli and E.faecalisAdditionaly monoculture of E.coli and E.faecalisAfterwards confirmation on ALOA®-Medium resp. Tryptic Soy agar |
| Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)) | ≤ 100 |
| Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)) | ≤ 100 |
| Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) | ≥ 1000 |
| Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) | ≥ 1000 |
| Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | ≥ 1000 |
| Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | ≥ 1000 |
| Growth on ALOA® medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)) | > 10 colonies |
| Growth on ALOA® medium(Listeria monocytogenes ATCC 35152 (WDCM 00109)) | > 10 colonies |
| Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021)) | blue green colonies with opaque halo |
| Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109)) | blue green colonies with opaque halo |
| Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)) | total inhibition |
| Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)) | total inhibition |
| Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | < 100 colonies |
| Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | < 100 colonies |
| Incubation:Half Fraser Broth: 24 ± 2 hours at 30 ± 1°CConfirmation on ALOA®-Medium: 44 ± 4 hours at 37 ± 1°CConfirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1°C |
